專利名稱:Electrophoresis system in series for multiple
agarose slab gels
發(fā)明人:Hamelin, Claude申請?zhí)枺篍P85109369.0申請日:19850725公開號:EP0173081A1公開日:19860305
專利附圖:
摘要:The disclosure describes an apparatus for the electrophoresis in series ofmultiple agarose slab gels. It comprises a train of at least three elongated box-likecontainers (1, 2, ... 12) such as twelve, which are set consecutively in substantially parallel
fashion at a variable distance from one another, each container to contain a runningbuffer (21, 22, 23, 24). The first and last containers (1, 12) are each provided with anelectrode (17,19) for connection to a single suitable power supply. A plurality of
horizontal plates (31, 33), each to contain a slab gel (35), are disposed in bridging fashionover adjacent spaced apart containers, to cover gaps between all the containers (1, 2,...12). The apparatus comprises first means (39) capable of establishing capillary contactbetween one end of the slab gel (35) and the running buffer which is present in a givencontainer; and second means (39) capable of establishing capillary contact between theother end of the slab gel (35) and the running buffer which is present in the nextcontainer. A method for the separation of components from a mixture of molecules ofmixed molecular weights, wherein a solution of said molecules is added to wells (37)formed in a horizontal slab gel (35) and the gel is thereafter treated by the aboveelectrophoresis to produce localized bands each being characteristic of a specificmolecular weight, is also disclosed.
申請人:INSTITUT ARMAND-FRAPPIER
地址:531 Boulevard des Prairies Laval Quebec H7N 4Z3 CA
國籍:CA
代理機(jī)構(gòu):Patentanw?lte Ruff, Beier, Sch?ndorf und Mütschele
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